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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been observed in several tumors, which leads to chromosome instability, and therefore might contribute to tumorigenesis. This protein was observed to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important purpose in selling mobile-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two further splice variants ended up reported but comprehensive nucleotide sequence information just isn't nevertheless offered.
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Blocking peptides are peptides that bind specifically to your goal antibody and block antibody binding. These peptide commonly has the epitope regarded through the antibody.
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This tutorial outlines additional probable optimization of cellular and immunoassay parameters to guarantee the best possible effects are...
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This guidebook outlines even more attainable optimization of mobile and immunoassay parameters to guarantee the absolute best effects are...
Antibodies sure to the blocking peptide no more bind on the epitope about the focus on protein. By comparing the staining from your blocked antibody here versus the antibody alone, you can see which staining is precise.
Focus on Data Cyclin E belongs for the very conserved cyclin family members, whose members are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities like a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is also degraded as cells progress by means of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by each antibodies...
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